The phylogenetic tree presented in the text was generated based on the ORF2 encoded RdRp, so the phylogenetic tree in the Figure S3 should be based on ORF1 rather than ORF2.But I still believe that the data of morphology, conidia and ITS sequence are enough to confirm that it is C. However, we have plan to publish the draft WGS in other research article. Actually, we already sent the genome for the draft whole genome sequencing, the data confirm that Ssa-44.1 is C. gloeosporioides. The data of conidia were added at the line 235-237. Results confirmed that strain Ssa-44.1 identify as C. We used morphological analysis, colony and conidia, together with ITS identification. For the fungus identification, the ITS is not sufficient to identify the pathogen to the species, although mango anthracnose is mainly caused by gloeosporioides.We have already arranged to send the article for proofreading. English writing needs to be improved because there are many small mistakes in the whole text.Therefore, this should be modified in line 352.Ĩ.Sequences of the Colletotrichum gloeosporioides RNA virus 2 should be provided as supplementary material if the information is unavailable in the database at present. Strictly speaking, the original strain, the eliminated strain, and the horizontally transferred virulent strain should be compared together.ħ.Only 17.60 + 2.08 % of the spores contained the virus, which should be considered to have low transfer efficiency. Considering that CgRV1 and CgRV2 shared the same host and more than 90% RdRp similarity, it may not be appropriate to classify them as distinct viral species.Ħ.For biological properties confirmation, the authors only compared the biological characteristics of the original strain Ssa-44.1 with those of the virus eliminated strain Ssa-44.1#18. Be careful to be consistent.ĥ.What concerned me most is the taxonomic status of the virus CgRV2 in this study, which is 88.22 % and 90.19 % similar to the ORF1 and ORF2 encoded amino acids of CgRV2, respectively. gloeosporioides strain Ssa-44.1 was measured as three replicates, however, this was showed as five replications in figure 4C. gloeosporioides.ģ.The phylogenetic tree presented in the text was generated based on the ORF2 encoded RdRp, so the phylogenetic tree in the Figure S3 should be based on ORF1 rather than ORF2.Ĥ.In 2.6.2, the vertical transmission rate of the virus in C. English writing needs to be improved because there are many small mistakes in the whole text.Ģ.For the fungus identification, the ITS is not sufficient to identify the pathogen to the species, although mango anthracnose is mainly caused by C. However, some concerns should be addressed.ġ. The content of this article could be of potential interest as it might expand our understanding of virus distribution and their effects on the important pathogenic fungus C. In addition, the virus was confirmed to be a hypovirulence associated mycovirus according to the virus-curing and biological analysis. The article of viruses-1847547 described the identification of a mycovirus from the phytopathogen Colletotrichum gloeosporioides through dsRNA extraction, genome cloning, sequence and phylogenetic analyses. We have made changes according to your comments. Here, I attached the revised version of the MS.Īuthor Response File: Author Response.pdf So, we re-considered changing the name of mycovirus from Colletotrichum gloeosporioides RNA virus 2 to Colletotrichum gloeosporioides RNA virus 1 strain Ssa-44.1 (CgRV1-Ssa-44.1). Therefore, the name of “CgRV2” should be re-considered.Īccording to your suggestion, we agree that they belong to the same species with different strains. Here I have a comment, authors could consider them.ĬgRV2 is very close to CgRV1 with more than 90% identity at RDRP, I think CgRV1 and CgRV2 should belong to the same species, and they are different strains. Authors got lots of results and supplied some new information on mycovirus, so I recommend publishing in Viruses. Authors confirmed that CgRV2 is related to hypovirulence and have potential virocontrol. Suharto et al reported a dsRNA mycovirus genome feature and their potential biological function in Colletotrichum gloeosporioides.
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